One game after leading the Wizards in scoring, Nene will sit out as Washington returns home to face the Milwaukee Bucks on Saturday night.
With the Wizards (41-38) completing a back-to-back game scenario, Nene will rest against the Bucks (15-64). The Brazilian big man has played two games since returning from a knee injury that forced him to miss 21 straight.
Nene scored 17 points in 20 minutes in Washington's 96-86 victory at the Orlando Magic on Friday.
Asked during his pregame media session if playing on consecutive nights would affect the Nene's status against the Bucks, Wizards coach Randy Wittman said "no." An hour later the team announced the power forward would sit with Chris Singleton suiting up as a member of the active roster.
The Wizards starting lineup remains the same: John Wall and Bradley Beal in the backcourt with Trevor Ariza and Trevor Booker at forward and Marcin Gortat at center.
Washington leads the season series 2-1, though the one loss came at the Verizon Center.
Kemba Walker (groin) will miss a second straight game for the Bobcats, who trail Washington by one game with three remaining for each team. Charlotte hosts the Philadelphia 76ers on Friday night. Washington regained sixth place in the Eastern Conference on Friday with a win at Orlando while the Walker-less Bobcats lost to the Boston Celtics.